Affiliation:
1. Sections of Clinical Microbiology
2. Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany
3. Molecular Pathology, Cleveland Clinic Foundation, Cleveland, Ohio
Abstract
ABSTRACT
The efficiencies of five commercially available nucleic acid extraction methods were evaluated for the recovery of a standardized inoculum of
Legionella pneumophila
in respiratory specimens (sputum and bronchoalveolar lavage [BAL] specimens). The concentrations of
Legionella
DNA recovered from sputa with the automated MagNA Pure (526,200 CFU/ml) and NucliSens (171,800 CFU/ml) extractors were greater than those recovered with the manual methods (i.e., Roche High Pure kit [133,900 CFU/ml], QIAamp DNA Mini kit [46,380 CFU/ml], and ViralXpress kit [13,635 CFU/ml]). The rank order was the same for extracts from BAL specimens, except that for this specimen type the QIAamp DNA Mini kit recovered more than the Roche High Pure kit.
Publisher
American Society for Microbiology
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