Affiliation:
1. Unité des Agents Antibactériens, Institut Pasteur, Paris, France
Abstract
ABSTRACT
A multiplex PCR assay was developed for detection of the six types of glycopeptide resistance characterized in enterococci and for identification of
Enterococcus faecium
,
Enterococcus faecalis
,
Staphylococcus aureus
, and
Staphylococcus epidermidis
at the species level. Primers targeting the genes
vanA
,
vanB
,
vanC
,
vanD
,
vanE
,
vanG
, and
ddl
of
E. faecium
and
E. faecalis
and
nuc
of
S. aureus
and a chromosomal portion specific to
S. epidermidis
were designed to allow amplification of fragments with various sizes. This specific and sensitive technique allows detection of glycopeptide-resistant strains, in particular methicillin-resistant
S. aureus
, that may escape phenotype-based automated rapid methods.
Publisher
American Society for Microbiology
Cited by
232 articles.
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