High Interlaboratory Reproducibility of DNA Sequence-Based Typing of Bacteria in a Multicenter Study-Reference-Cited by-同舟云学术

High Interlaboratory Reproducibility of DNA Sequence-Based Typing of Bacteria in a Multicenter Study

Published:2006-02 Issue:2 Volume:44 Page:619-621
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Aires-de-Sousa M.¹,Boye K.²,de Lencastre H.¹,Deplano A.³,Enright M. C.⁴,Etienne J.⁵,Friedrich A.⁶,Harmsen D.⁷,Holmes A.⁸,Huijsdens X. W.⁹,Kearns A. M.⁸,Mellmann A.⁶,Meugnier H.⁵,Rasheed J. K.¹⁰,Spalburg E.⁹,Strommenger B.¹¹,Struelens M. J.³,Tenover F. C.¹⁰,Thomas J.⁴,Vogel U.¹²,Westh H.²,Xu J.¹³,Witte W.¹¹

Affiliation:

1. Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal

2. Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark

3. Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium

4. Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom

5. Laboratoire Central de Microbiologie, Hôpital Edouard Herriot, Lyon, France

6. Institut für Hygiene, University Hospital Münster, Münster, Germany

7. Department of Periodontology, University Hospital Münster, Münster, Germany

8. Center for Infections, Health Protection Agency, London, United Kingdom

9. Diagnostic Laboratory for Infectious Diseases, National Institute of Public Health and the Environment, Bilthoven, The Netherlands

10. Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia

11. Robert-Koch-Institut, Wernigerode, Germany

12. Institut für Hygiene und Microbiologie, University Hospital Würzburg, Würzburg, Germany

13. State Key Laboratory of Infectious Diseases Prevention and Control, National Institute for Communicable Disease Prevention and Control, Beijing, People's Republic of China

Abstract

ABSTRACT Current DNA amplification-based typing methods for bacterial pathogens often lack interlaboratory reproducibility. In this international study, DNA sequence-based typing of the Staphylococcus aureus protein A gene ( spa , 110 to 422 bp) showed 100% intra- and interlaboratory reproducibility without extensive harmonization of protocols for 30 blind-coded S. aureus DNA samples sent to 10 laboratories. Specialized software for automated sequence analysis ensured a common typing nomenclature.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.44.2.619-621.2006

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