First Report of Klebsiella oxytoca Strain Coproducing KPC-2 and IMP-8 Carbapenemases

Abstract

ABSTRACTThe study shows for the first time the presence of theKlebsiella oxytocastrain fp10 coproducing plasmid-mediated KPC-2 and IMP-8 carbapenemases. The strain was obtained from the fecal sample of an inpatient and showed high-level resistance to imipenem and ertapenem (MICs > 32 μg/ml). Conjugation experiments demonstrated the transferability of the carbapenem-resistant determinants. The results of plasmid analysis and Southern hybridization revealed that theblaKPC-2gene was located on transferable plasmid pFP10-1 (∼54 kb), whereas theblaIMP-8gene was on transferable plasmid pFP10-2 (∼180 kb). Analysis of the genetic environment of these two genes has demonstrated that ISKpn6and ISKpn8are involved in the spread of theblaKPC-2gene, while the transposable elements IS26,intI1, andtniCmight contribute to the dissemination of theblaIMP-8gene. The chimera of several transposon-associated elements indicated a novel genetic environment of IMP-type metallo-β-lactamase gene inEnterobacteriaceaefrom China.