Detection of Shiga-Like Toxin ( stx 1 and stx 2 ), Intimin ( eaeA ), and Enterohemorrhagic Escherichia coli (EHEC) Hemolysin (EHEC hlyA ) Genes in Animal Feces by Multiplex PCR-Reference-Cited by-同舟云学术

Detection of Shiga-Like Toxin ( stx 1 and stx 2 ), Intimin ( eaeA ), and Enterohemorrhagic Escherichia coli (EHEC) Hemolysin (EHEC hlyA ) Genes in Animal Feces by Multiplex PCR

Published:1999-02 Issue:2 Volume:65 Page:868-872
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Fagan Peter K.¹,Hornitzky Michael A.¹,Bettelheim Karl A.²,Djordjevic Steven P.¹

Affiliation:

1. Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, Camden, New South Wales 2570,1 and

2. Victorian Infectious Diseases Reference Laboratory, Fairfield Hospital, Fairfield, Victoria 3078,2 Australia

Abstract

ABSTRACT A multiplex PCR was developed for the rapid detection of genes encoding Shiga toxins 1 and 2 ( stx 1 and stx 2 ), intimin ( eaeA ), and enterohemolysin A ( hlyA ) in 444 fecal samples derived from healthy and clinically affected cattle, sheep, pigs, and goats. The method involved non-solvent-based extraction of nucleic acid from an aliquot of an overnight culture of feces in EC (modified) broth. The detection limit of the assay for both fecal samples and pure cultures was between 18 and 37 genome equivalents. stx 1 and hlyA were the most commonly encountered virulence factors.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.2.868-872.1999

Reference32 articles.

1. Which Shiga toxin-producing types of Escherichia coli are important?;Acheson D. W.;ASM News,1996

2. Characterization of the eaeA gene from rabbit enteropathogenic Escherichia coli strain RDEC-1 and comparison to other eaeA genes from bacteria that cause attaching-effacing lesions.;Agin T. S.;FEMS Microbiol. Lett.,1996

3. Emerging food borne pathogens: Escherichia coli O157:H7 as a model of entry of a new pathogen into the food supply of the developed world.;Armstrong G. L.;Epidemiol. Rev.,1996

4. Enterohaemorrhagic Escherichia coli—a review.;Bettelheim K. A.;Int. Food Hyg.,1996

5. Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals

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