Development and Clinical Validation of a Pangenotypic PCR-Based Assay for the Detection and Quantification of Hepatitis E Virus ( Orthohepevirus A Genus)-Reference-Cited by-同舟云学术

Development and Clinical Validation of a Pangenotypic PCR-Based Assay for the Detection and Quantification of Hepatitis E Virus ( Orthohepevirus A Genus)

Published:2021-01-21 Issue:2 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Frías Mario¹,López-López Pedro¹,Zafra Ismael¹,Caballero-Gómez Javier¹²,Machuca Isabel¹,Camacho Ángela¹,Risalde María A.³,Rivero-Juárez Antonio¹^ORCID,Rivero Antonio¹

Affiliation:

1. Virología Clínica y Zoonosis, Hospital Universitario Reina Sofía de Córdoba, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Universidad de Córdoba, Córdoba, Spain

2. Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain

3. Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain

Abstract

The objective of this study was to design a pangenotypic PCR-based assay for the detection and quantification of hepatitis E virus (HEV) RNA from across the entire spectrum of described genotypes belonging to the Orthohepevirus A genus. The optimal conditions and the performance of the assay were determined by testing the WHO standard strain (6219/10) and the WHO HEV panel (8578/13). Similarly, performance comparisons were made with two commercial assays (Real Star HEV RT-PCR 2.0 and ampli Cube HEV 2.

Funder

Red de Investigacion de SIDA en España, Instituto Carlos III

Ministerio de Educacion, Cultura y Deportes

Consejeria de Salud de la Junta de Andalucia

Fundacion para la investigacion en Salud. Instituto Carlos III

Ministerio de Ciencia, Innovación y Universidades

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02075-20