Clinical comparison of the Isolator 1.5 microbial tube and the BACTEC radiometric system for detection of bacteremia in children

Author:

Carey R B

Abstract

The Isolator 1.5 microbial tube (E. I. du Pont de Nemours & Co., Inc., Wilmington , Del.) was compared with the BACTEC radiometric detection system (Johnston Laboratories, Inc., Cockeysville, Md.) for the detection of bacteremia in children. The Isolator 1.5 is a blood culture system designed for small volumes of blood (0.5 to 1.5 ml). The method involves lysis of the cells of the patient and the direct plating of the entire blood lysate on agar media appropriate for the growth of fastidious microorganisms. Of 1,500 paired samples inoculated into the two systems, 68 were positive for 73 clinically significant organisms. The Isolator 1.5 recovered 81% of the positive cultures compared with 84% recovered by the BACTEC system. When paired blood samples with disproportionate volumes were excluded, the Isolator 1.5 detected 3% more positive cultures. More isolates of Streptococcus pneumoniae and Neisseria meningitidis were recovered by the Isolator 1.5, whereas Haemophilus influenzae was recovered most often in the BACTEC bottles (P greater than 0.1). The contamination rates were 8.7 and 3.1% for the Isolator 1.5 and the BACTEC system, respectively. In cultures positive by both systems, the mean time to detection was 4.1 h faster with the Isolator 1.5. The mean time to obtain isolated colonies was 26.6 h faster with the Isolator 1.5. These data indicate the potential value of the Isolator 1.5 microbial tube as a simple, rapid, and sensitive method for the detection of bacteremia in children.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

1. False no-growth blood cultures in pneumococcal pneumonia;Adeniyi-Jones C. C.;J. Clin. Microbiol.,1980

2. Evaluation of a Iysis-centrifugation system for recovery of yeasts and filamentous fungi from blood;Bille J.;J. Clin. Microbiol.,1983

3. Blood culture technique based on centrifugation: clinical evaluation;Dorn G. L.;J. Clin. Microbiol.,1976

4. Improved blood culture technique based on centrifugation: clinical evaluation;Dorn G. L.;J. Clin. Microbiol.,1979

5. New centrifugation blood culture device;Dorn G. L.;J. Clin. Microbiol.,1978

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3