Affiliation:
1. Special Project Unit, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada
Abstract
ABSTRACT
A multiplex PCR assay for detection of genes for staphylococcal enterotoxins A to E (
entA
,
entB
,
entC
,
entD
, and
entE
), toxic shock syndrome toxin 1 (
tst
), exfoliative toxins A and B (
etaA
and
etaB
), and intrinsic methicillin resistance (
mecA
) was developed. Detection of
femA
was used as an internal positive control. The multiplex PCR assay combined the primers for
sea
to
see
and
femA
in one set and those for
eta
,
etb
,
tst
,
mecA
, and
femA
in the other set. Validation of the assay was performed using 176 human isolates of
Staphylococcus aureus
. This assay offers a very specific, quick, reliable, and inexpensive alternative to conventional PCR assays used in clinical laboratories to identify various staphylococcal toxin genes.
Publisher
American Society for Microbiology
Cited by
519 articles.
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