Diagnosis of Enterovirus Infection by Genus-Specific PCR and Enzyme-Linked Immunosorbent Assays-Reference-Cited by-同舟云学术

Diagnosis of Enterovirus Infection by Genus-Specific PCR and Enzyme-Linked Immunosorbent Assays

Published:2003-02 Issue:2 Volume:41 Page:841-844
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Craig Maria E.¹²³,Robertson Peter⁴,Howard Neville J.¹,Silink Martin¹,Rawlinson William D.²⁵

Affiliation:

1. The Children's Hospital at Westmead, Sydney

2. Virology Research Laboratory, Virology Division

3. School of Women’s and Children’s Health

4. Area Serology Laboratory, Department of Microbiology, South Eastern Area Laboratory Services, Prince of Wales Hospital, Randwick

5. Schools of Medical Sciences, Biochemistry and Biomedical Sciences, University of New South Wales, Kensington, New South Wales, Australia

Abstract

ABSTRACT PCR for the diagnosis of enterovirus infections is resource intensive but is increasingly used due to wide availability. Enzyme-linked immunosorbent assays (ELISAs) that detect heterotypical antibodies against enterovirus immunoglobulin M (IgM), IgA, and IgG were compared with reverse transcription-PCR by using primers specific to the 5′ untranslated regions of 60 enterovirus species. The ELISAs were less sensitive than the PCR, and only the ELISA for IgM was highly specific. When retrospective diagnosis is important or when specimens are unsuitable for PCR, the ELISA has a limited role if PCR is not available.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.41.2.841-844.2003

Reference25 articles.

1. Comparison of Use of Cerebrospinal Fluid, Serum, and Throat Swab Specimens in Diagnosis of Enteroviral Acute Neurological Infection by a Rapid RNA Detection PCR Assay

2. Boman, J., B. Nilsson, and P. Juto. 1992. Serum IgA, IgG, and IgM responses to different enteroviruses as measured by a coxsackie B5-based indirect ELISA. J. Med. Virol.38:32-35.

3. Craig, M. E., T. Vale, P. Robertson, W. D. Rawlinson, and B. Gould. 1999. Enterovirus 71 infection in Australian expatriate children following an outbreak in Malaysia. J. Paediatr. Child Health35:107-108.

4. Immunoglobulin M capture immunoassay in investigation of coxsackievirus B5 and B6 outbreaks in South Australia

5. Hawkes R. A. 1979. General principles underlying laboratory diagnosis of viral infections p. 24-26. In E. H. Lennette and N. J. Schmidt (ed.) Diagnostic procedures for viral rickettsial and chlamidyal infections. American Public Health Association Washington D.C.

Cited by 32 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. The clinical epidemiology, management, and outcomes of patients diagnosed with encephalitis in North Carolina, 2015–2020;Journal of Clinical Microbiology;2023-12-19

2. Epidemiological and molecular characterization of Coxsackievirus A6 causing hand, foot, and mouth disease in the Philippines, 2012–2017;Infection, Genetics and Evolution;2023-10

3. Enterovirus detection in different regions of Madagascar reveals a higher abundance of enteroviruses of species C in areas where several outbreaks of vaccine-derived polioviruses occurred;BMC Infectious Diseases;2022-11-08

4. Molecular typing of enteroviruses: comparing 5′UTR, VP1 and whole genome sequencing methods;Pathology;2022-10

5. Viruses and Type 1 Diabetes: From Enteroviruses to the Virome;Microorganisms;2021-07-16