Competitive Fitness of Essential Gene Knockdowns Reveals a Broad-Spectrum Antibacterial Inhibitor of the Cell Division Protein FtsZ-Reference-Cited by-同舟云学术

Competitive Fitness of Essential Gene Knockdowns Reveals a Broad-Spectrum Antibacterial Inhibitor of the Cell Division Protein FtsZ

Published:2018-12 Issue:12 Volume:62 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Hogan Andrew M.¹,Scoffone Viola C.²,Makarov Vadim³,Gislason April S.¹,Tesfu Haben¹,Stietz Maria S.¹,Brassinga Ann Karen C.¹,Domaratzki Michael⁴,Li Xuan⁵,Azzalin Alberto²⁶,Biggiogera Marco²,Riabova Olga³,Monakhova Natalia³,Chiarelli Laurent R.²^ORCID,Riccardi Giovanna²,Buroni Silvia²,Cardona Silvia T.¹⁷^ORCID

Affiliation:

1. Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada

2. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

3. Federal Research Center Fundamentals of Biotechnology of the Russian Academy of Sciences, Moscow, Russia

4. Department of Computer Science, University of Manitoba, Winnipeg, Manitoba, Canada

5. Department of Mathematics and Statistics, University of Minnesota-Duluth, Duluth, Minnesota, USA

6. Department of Internal Medicine and Therapeutics, University of Pavia, Fondazione IRCCS San Matteo, Pavia, Italy

7. Department of Medical Microbiology and Infectious Disease, University of Manitoba, Winnipeg, Canada

Abstract

To streamline the elucidation of antibacterial compounds’ mechanism of action, comprehensive high-throughput assays interrogating multiple putative targets are necessary. However, current chemogenomic approaches for antibiotic target identification have not fully utilized the multiplexing potential of next-generation sequencing.

Funder

Italian Cystic Fibrosis Foundation

Cystic Fibrosis Canada

Gouvernement du Canada | Canadian Institutes of Health Research

Research Manitoba

Cystic Fibrosis Foundation

Università degli Studi di Pavia

University of Manitoba

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology