Use of Dipsticks for Rapid Diagnosis of Cholera Caused by Vibrio cholerae O1 and O139 from Rectal Swabs

Author:

Bhuiyan N. A.1,Qadri Firdausi1,Faruque A. S. G.1,Malek M. A1,Salam M. A.1,Nato Farida2,Fournier J. M.2,Chanteau S.3,Sack David A.1,Balakrish Nair G.1

Affiliation:

1. Laboratory and Clinical Sciences Divisions, International Centre for Diarrhoeal Diseases Research, Bangladesh (ICDDR,B), Dhaka 1212, Bangladesh

2. Institut Pasteur, Paris, France

3. CERMES, Niamey, Niger

Abstract

ABSTRACT We evaluated the recently developed dipsticks for the rapid detection of Vibrio cholerae serotypes O1 and O139 from rectal swabs of hospitalized diarrheal patients after enrichment for 4 h in alkaline peptone water. The sensitivity and specificity of the dipsticks were above 92 and 91%, respectively. The dipsticks represent the first rapid test which has been successfully used to diagnose cholera from rectal swabs, and this would immensely improve surveillance for cholera, especially in remote settings.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference13 articles.

1. Characterization of Aeromonas trota strains that cross-react with Vibrio cholerae O139 Bengal

2. Ansaruzzaman, M., T. Shimada, N. A. Bhuiyan, S. Nahar, M. S. Islam, and M. J. Albert. 1999. Cross-reaction between a strain of Vibrio mimicus and V. cholerae O139 Bengal. J. Med. Microbiol.48:873-877.

3. Bik, E. M., A. E. Bunschoten, R. D. Gouw, and F. R. Mooi. 1995. Genesis of the novel epidemic Vibrio cholerae O139 strain: evidence for horizontal transfer of genes involved in polysaccharide synthesis. EMBO J.14:209-216.

4. Faruque, S. M., D. A. Sack, R. B. Sack, R. R. Colwell, Y. Takeda, and G. B. Nair. 2003. Emergence and evolution of Vibrio cholerae. Proc. Natl. Acad. Sci. USA100:1304-1309.

5. Faruque S. M. N. Chowdhury M. Kamruzzaman Q. S. Ahmad A. S. G. Faruque M. A. Salam T. Ramamurthy G. B. Nair A. Weintraub and D. A. Sack. 2002. Analysis of reemergent Vibrio cholerae O139 strains associated with an epidemic of cholera in Bangladesh p. 11-15. In Proceedings of the 37th US-Japan Joint Conference on Cholera and Other Bacterial Enteric Infections Panel December 17-19 2002 Naha Okinawa Japan. US-Japan Cooperative Medical Science Program Bethesda Md.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3