A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine-Reference-Cited by-同舟云学术

A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine

Published:2012-06 Issue:6 Volume:50 Page:2072-2074
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Koizumi Nobuo¹,Nakajima Chie²,Harunari Tsunehito³,Tanikawa Tsutomu³,Tokiwa Toshihiro⁴,Uchimura Eriko⁵,Furuya Tokujiro⁶,Mingala Claro Niegos⁷,Villanueva Marvin Ardeza⁷,Ohnishi Makoto¹,Suzuki Yasuhiko²

Affiliation:

1. Department of Bacteriology, National Institute of Infectious Disease, Tokyo, Japan

2. Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Hokkaido, Japan

3. Ikari Corporation, Chiba, Japan

4. Section of Environmental Parasitology, Graduate School of Tokyo Medical and Dental University, Tokyo, Japan

5. Kagoshima Central Livestock Hygiene Service Center, Kagoshima, Japan

6. Pfizer Japan Inc., Tokyo, Japan

7. Animal Health Unit, Philippine Carabao Center National Headquarters and Genepool, Nueva Ecija, Philippines

Abstract

ABSTRACT We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs , a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00481-12

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