Affiliation:
1. Service de Bactériologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre 94275, and Faculté de Médecine Paris-Sud, Paris, France
2. CIDEIM (International Center for Medical Research and Training), Cali, Colombia
3. Stroger Hospital of Cook County and Chicago Infectious Disease Research Institute, Chicago, Illinois
Abstract
ABSTRACT
Genetic structures surrounding the carbapenem-hydrolyzing Ambler class A
bla
KPC
gene were characterized in several KPC-positive
Klebsiella pneumoniae
and
Pseudomonas aeruginosa
strains isolated from the United States, Colombia, and Greece. The
bla
KPC
genes were associated in all cases with transposon-related structures. In the
K. pneumoniae
YC isolate from the United States, the β-lactamase
bla
KPC-2
gene was located on a novel Tn
3
-based transposon, Tn
4401
. Tn
4401
was 10 kb in size, was delimited by two 39-bp imperfect inverted repeat sequences, and harbored, in addition to the β-lactamase
bla
KPC-2
gene, a transposase gene, a resolvase gene, and two novel insertion sequences, IS
Kpn6
and IS
Kpn7
. Tn
4401
has been identified in all isolates. However, two isoforms of this transposon were found: Tn
4401
a was found in
K. pneumoniae
YC and
K. pneumoniae
GR from the United States and Greece, respectively, and differed by a 100-bp deletion, located just upstream of the
bla
KPC-2
gene, compared to the sequence of Tn
4401
b, which was found in the Colombian isolates. In all isolates tested, Tn
4401
was flanked by a 5-bp target site duplication, the signature of a recent transposition event, and was inserted in different open reading frames located on plasmids that varied in size and nature. Tn
4401
is likely at the origin of carbapenem-hydrolyzing β-lactamase KPC mobilization to plasmids and its further insertion into various-sized plasmids identified in nonclonally related
K. pneumoniae
and
P. aeruginosa
isolates.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
412 articles.
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