Direct Detection and Identification of Prosthetic Joint Infection Pathogens in Synovial Fluid by Metagenomic Shotgun Sequencing-Reference-Cited by-同舟云学术

Direct Detection and Identification of Prosthetic Joint Infection Pathogens in Synovial Fluid by Metagenomic Shotgun Sequencing

Published:2018-09 Issue:9 Volume:56 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ivy Morgan I.¹,Thoendel Matthew J.²,Jeraldo Patricio R.³^ORCID,Greenwood-Quaintance Kerryl E.¹,Hanssen Arlen D.⁴,Abdel Matthew P.⁴,Chia Nicholas³,Yao Janet Z.³,Tande Aaron J.²,Mandrekar Jayawant N.⁵,Patel Robin¹²^ORCID

Affiliation:

1. Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA

2. Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, Minnesota, USA

3. Department of Surgery, Mayo Clinic, Rochester, Minnesota, USA

4. Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota, USA

5. Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic, Rochester, Minnesota, USA

Abstract

Metagenomic shotgun sequencing has the potential to transform how serious infections are diagnosed by offering universal, culture-free pathogen detection. This may be especially advantageous for microbial diagnosis of prosthetic joint infection (PJI) by synovial fluid analysis since synovial fluid cultures are not universally positive and since synovial fluid is easily obtained preoperatively.

Funder

HHS | National Institutes of Health

HHS | NIH | National Institute of Allergy and Infectious Diseases

HHS | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00402-18

Reference46 articles.

1. Epidemiology and New Developments in the Diagnosis of Prosthetic Joint Infection