Affiliation:
1. Institute of Molecular Biology, University of Oregon, Eugene 97403.
Abstract
Wild-type Escherichia coli are resistant to genetic transformation by purified linear DNA, probably in part because of exonuclease activity. We demonstrate that E. coli containing a recD mutation could be easily transformed by linearized plasmids containing a selectable marker. The marker was transferred to the chromosome by homologous recombination, whereas plasmid markers not in the region of homology were lost.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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