Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19-Reference-Cited by-同舟云学术

Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19

Published:2020-12-17 Issue:1 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Babiker Ahmed¹²^ORCID,Bradley Heath L.²,Stittleburg Victoria D.¹,Ingersoll Jessica M.²,Key Autum²,Kraft Colleen S.¹²^ORCID,Waggoner Jesse J.¹,Piantadosi Anne¹²^ORCID

Affiliation:

1. Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA

2. Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA

Abstract

Broad testing for respiratory viruses among persons under investigation (PUIs) for SARS-CoV-2 has been performed inconsistently, limiting our understanding of alternative viral infections and coinfections in these patients. RNA metagenomic next-generation sequencing (mNGS) offers an agnostic tool for the detection of both SARS-CoV-2 and other RNA respiratory viruses in PUIs. Here, we used RNA mNGS to assess the frequencies of alternative viral infections in SARS-CoV-2 RT-PCR-negative PUIs ( n = 30) and viral coinfections in SARS-CoV-2 RT-PCR-positive PUIs ( n = 45).

Funder

Doris Duke Charitable Foundation

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

Center for AIDS Research, Emory University

Emory University | School of Medicine, Emory University

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02142-20

Reference16 articles.