Validation of Fourier Transform Infrared Spectroscopy for Serotyping of Streptococcus pneumoniae

Author:

Passaris I.1ORCID,Mauder N.2,Kostrzewa M.2,Burckhardt I.3,Zimmermann S.3ORCID,van Sorge N. M.45,Slotved H.-C.6,Desmet S.78ORCID,Ceyssens P.-J.1

Affiliation:

1. Bacterial Diseases Unit, Sciensano, Brussels, Belgium

2. Bruker Daltonic GmbH, Bremen, Germany

3. Department of Infectious Diseases, Microbiology and Hygiene, University Hospital of Heidelberg, Heidelberg, Germany

4. Department of Medical Microbiology and Infection Prevention, UMC Amsterdam, University of Amsterdam, Amsterdam, The Netherlands

5. Netherlands Reference Laboratory for Bacterial Meningitis, UMC Amsterdam, University of Amsterdam, Amsterdam, The Netherlands

6. Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen, Denmark

7. National Reference Centre for (invasive) S. pneumoniae, UZ Leuven, Leuven, Belgium

8. Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium

Abstract

Fourier transform infrared (FT-IR) spectroscopy (IR Biotyper; Bruker) allows highly discriminatory fingerprinting of closely related bacterial strains. In this study, FT-IR spectroscopy-based capsular typing of Streptococcus pneumoniae was validated as a rapid, cost-effective, and medium-throughput alternative to the classical phenotypic techniques.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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