Subcellular Detection of SARS-CoV-2 RNA in Human Tissue Reveals Distinct Localization in Alveolar Type 2 Pneumocytes and Alveolar Macrophages-Reference-Cited by-同舟云学术

Subcellular Detection of SARS-CoV-2 RNA in Human Tissue Reveals Distinct Localization in Alveolar Type 2 Pneumocytes and Alveolar Macrophages

Published:2022-02-22 Issue:1 Volume:13 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Acheampong Kofi K.¹,Schaff Dylan L.²,Emert Benjamin L.³,Lake Jonathan¹,Reffsin Sam²,Shea Emily K.⁴,Comar Courtney E.¹⁵⁶⁷⁸,Litzky Leslie A.¹,Khurram Nigar A.¹⁹,Linn Rebecca L.¹¹⁰,Feldman Michael¹,Weiss Susan R.⁵⁶^ORCID,Montone Kathleen T.¹,Cherry Sara¹⁶¹¹,Shaffer Sydney M.¹²^ORCID

Affiliation:

1. Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

2. Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, Pennsylvania, USA

3. Genomics and Computational Biology Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

4. Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

5. Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania, USA

6. Penn Center for Research on Coronaviruses and Other Emerging Pathogens, Philadelphia, Pennsylvania, USA

7. Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, USA

8. Infectious Disease Diagnostics Laboratory, The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA

9. Department of Pathology, Northwestern University, Feinberg School of Medicine, Chicago, Illinois, USA

10. Division of Anatomic Pathology, The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA

11. Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, USA

Abstract

Here, we developed an in situ RNA detection assay for RNA generated by the SARS-CoV-2 virus. We found viral RNA in lung, lymph node, and placenta samples from pathology specimens from COVID patients. Using high-magnification microscopy, we can visualize the subcellular distribution of these RNA in single cells.

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mbio.03751-21

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