Real-Time Detection of Virus Particles and Viral Protein Expression with Two-Color Nanoparticle Probes-Reference-Cited by-同舟云学术

Real-Time Detection of Virus Particles and Viral Protein Expression with Two-Color Nanoparticle Probes

Published:2005-07 Issue:13 Volume:79 Page:8625-8628
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Agrawal Amit¹,Tripp Ralph A.²,Anderson Larry J.³,Nie Shuming¹

Affiliation:

1. Departments of Biomedical Engineering and Chemistry, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001

2. College of Veterinary Medicine, Department of Infectious Diseases, University of Georgia, Athens, Georgia

3. Centers for Disease Control and Prevention, National Center for Infectious Diseases, Division of Respiratory and Enteric Viruses, Atlanta

Abstract

ABSTRACT Respiratory syncytial virus (RSV) mediates serious lower respiratory tract illness in infants and young children and is a significant pathogen of the elderly and immune compromised. Rapid and sensitive RSV diagnosis is important to infection control and efforts to develop antiviral drugs. Current RSV detection methods are limited by sensitivity and/or time required for detection. In this study, we show that antibody-conjugated nanoparticles rapidly and sensitively detect RSV and estimate relative levels of surface protein expression. A major development is use of dual-color quantum dots or fluorescence energy transfer nanobeads that can be simultaneously excited with a single light source.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.79.13.8625-8628.2005

Reference18 articles.

1. Anonymous. 2002. Respiratory syncytial virus activity—United States, 2000-01 season. Morb. Mortal. Wkly. Rep.51:26-28.

2. Castro, A., and J. G. Williams. 1997. Single-molecule detection of specific nucleic acid sequences in unamplified genomic DNA. Anal. Chem.69:3915-3920.

3. Chan, W. C. W., and S. M. Nie. 1998. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science281:2016-2018.

4. Couch, R. B., J. A. Englund, and E. Whimbey. 1997. Respiratory viral infections in immunocompetent and immunocompromised persons. Am. J. Med.102:2-9, 25-26.

5. Falsey, A. R. 1998. Respiratory syncytial virus infection in older persons. Vaccine16:1775-1778.

Cited by 82 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Geiger Mode Single Photon Counting: A Laboratory Experiment Exploring Delayed Fluorescence in Plants;Journal of Chemical Education;2023-09-13

2. Quantum Dots in Biomedical Applications: Recent Advancements and Future Prospects;Carbon Nanostructures in Biomedical Applications;2023

3. Quantum dots in the biomedical world: A smart advanced nanocarrier for multiple venues application;Archiv der Pharmazie;2022-09-04

4. Pathogen identification through surface marker recognition methods;Handbook of Microbial Nanotechnology;2022

5. Applications of Nanotechnology in Pulmonary Disease Diagnosis;Advanced Drug Delivery Strategies for Targeting Chronic Inflammatory Lung Diseases;2022