Affiliation:
1. Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032
Abstract
ABSTRACT
The enzyme CwlJ is involved in the depolymerization of cortex peptidoglycan during germination of spores of
Bacillus subtilis
. CwlJ with a C-terminal His tag was functional and was extracted from spores by procedures that remove spore coat proteins. However, this CwlJ was not extracted from disrupted spores by dilute buffer, high salt concentrations, Triton X-100, Ca
2+
-dipicolinic acid, dithiothreitol, or peptidoglycan digestion, disappeared during spore germination, and was not present in
cotE
spores in which the spore coat is aberrant. These findings indicate the following: (i) the reason decoated and
cotE
spores germinate poorly with dipicolinic acid is the absence of CwlJ from these spores; and (ii) CwlJ is located in the spore coat, presumably tightly associated with one or more other coat proteins.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
96 articles.
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