Mechanism of Cooperative Degradation of Gum Arabic Arabinogalactan Protein by Bifidobacterium longum Surface Enzymes-Reference-Cited by-同舟云学术

Mechanism of Cooperative Degradation of Gum Arabic Arabinogalactan Protein by Bifidobacterium longum Surface Enzymes

Published:2022-03-22 Issue:6 Volume:88 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Sasaki Yuki¹,Komeno Masahiro²,Ishiwata Akihiro³^ORCID,Horigome Ayako⁴,Odamaki Toshitaka⁴^ORCID,Xiao Jin-Zhong⁴,Tanaka Katsunori³⁵,Ito Yukishige³⁶,Kitahara Kanefumi¹⁷,Ashida Hisashi²⁸,Fujita Kiyotaka¹⁷^ORCID

Affiliation:

1. The United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Kagoshima, Japan

2. Graduate School of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan

3. RIKEN Cluster for Pioneering Research, Wako, Saitama, Japan

4. Next Generation Science Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa, Japan

5. Department of Chemical Science and Engineering, Tokyo Institute of Technology, Meguro, Tokyo, Japan

6. Graduate School of Science, Osaka University, Toyonaka, Osaka, Japan

7. Faculty of Agriculture, Kagoshima University, Kagoshima, Kagoshima, Japan

8. Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan

Abstract

Terminal galactose residues of the side chain of gum arabic arabinogalactan protein (AGP) are mainly substituted by α1,3/α1,4-linked Ara f and β1,6-linked α- l -Rha p -(1→4)-β- d -Glc p A residues. This study found a multidomain BlArafE with GH43_22 and GH43_34 catalytic domains showing cooperative action for degrading α1,3/α1,4-linked Ara f of the side chain of gum arabic AGP.

Funder

Japan Society for the Promotion of Science (JSPS) Kakenhi

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.02187-21

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