Double-Staining Method for Differentiation of Morphological Changes and Membrane Integrity of Campylobacter coli Cells-Reference-Cited by-同舟云学术

Double-Staining Method for Differentiation of Morphological Changes and Membrane Integrity of Campylobacter coli Cells

Published:2002-10 Issue:10 Volume:68 Page:5151-5154
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Alonso Jose L.¹,Mascellaro Salvatore²,Moreno Yolanda²,Ferrús María A.²,Hernández Javier²

Affiliation:

1. Instituto de Hidrología y Medio Natural

2. Departamento de Biotecnología, Universidad Politécnica, 46022 Valencia, Spain

Abstract

ABSTRACT We developed a double-staining procedure involving NanoOrange dye (Molecular Probes, Eugene, Oreg.) and membrane integrity stains (LIVE/DEAD Bac Light kit; Molecular Probes) to show the morphological and membrane integrity changes of Campylobacter coli cells during growth. The conversion from a spiral to a coccoid morphology via intermediary forms and the membrane integrity changes of the C. coli cells can be detected with the double-staining procedure. Our data indicate that young or actively growing cells are mainly spiral shaped (green-stained cells), but older cells undergo a degenerative change to coccoid forms (red-stained cells). Club-shaped transition cell forms were observed with NanoOrange stain. Chlorinated drinking water affected the viability but not the morphology of C. coli cells.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.10.5151-5154.2002

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