Cloning and Characterization of the Crystal Protein-Encoding Gene of Bacillus thuringiensis subsp. yunnanensis-Reference-Cited by-同舟云学术

Cloning and Characterization of the Crystal Protein-Encoding Gene of Bacillus thuringiensis subsp. yunnanensis

Published:2002-01 Issue:1 Volume:68 Page:408-411
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Balasubramanian Periasamy¹,Jayakumar R.¹,Shambharkar Prashant¹,Unnamalai N.¹,Pandian S. Karutha¹,Kumaraswami N. Selvamuthu¹,Ilangovan R.¹,Sekar Vaithilingam¹

Affiliation:

1. Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, India

Abstract

ABSTRACT Molecular cloning and characterization of a novel cry gene, cry32Aa , of Bacillus thuringiensis subsp. yunnanensis was carried out. The Cry32Aa protein was predicted to have a molecular mass of 139.2 kDa and was found to have an unusual 42-amino-acid-long tail at the C terminus. The cry32Aa gene was localized on the 103-MDa plasmid of the organism. Bioassays showed no toxicity against several moths and mosquitoes. However, it exhibited weak toxicity against larvae of the diamondback moth, Plutella xylostella .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.1.408-411.2002

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