In Vivo Evolution of CTX-M-215, a Novel Narrow-Spectrum β-Lactamase in an Escherichia coli Clinical Isolate Conferring Resistance to Mecillinam-Reference-Cited by-同舟云学术

In Vivo Evolution of CTX-M-215, a Novel Narrow-Spectrum β-Lactamase in an Escherichia coli Clinical Isolate Conferring Resistance to Mecillinam

Published:2020-10-20 Issue:11 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Yin Mengyun¹²,Hu Guoping³,Shen Zhen⁴,Fang Chengli⁵⁶,Zhang Xuefei¹²,Li Dan¹²,Doi Yohei⁷,Zhang Yu⁵⁶,Wang Minggui¹²,Guo Qinglan¹²^ORCID

Affiliation:

1. Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, China

2. Key Laboratory of Clinical Pharmacology of Antibiotics, National Heath Commission of the People's Republic of China, Shanghai, China

3. WuXi AppTec Co., Ltd., Shanghai, China

4. Department of Laboratory, Renji Hospital, Shanghai Jiaotong University, Shanghai, China

5. The Key Laboratory of Synthetic Biology, Chinese Academy of Sciences, Shanghai, China

6. CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China

7. Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA

Abstract

Here, we report a novel narrow-spectrum β-lactamase CTX-M-215 identified in an Escherichia coli clinical isolate in China and conferring high-level resistance to mecillinam but not to cefotaxime. CTX-M-215 differed from CTX-M-125, a CTX-M extended-spectrum β-lactamase (ESBL), by an N132D substitution, which decreased hydrolytic activities toward penicillins and cephalosporins except for mecillinam. High similarity was observed between CTX-M-215- and CTX-M-125-bearing plasmids, carried by different isolates in the same patient, indicating in vivo evolution of CTX-M-215 from CTX-M-125.

Funder

Shanghai Pujiang Program

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00562-20

Reference20 articles.

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