Monitoring the Dissemination of the Broad-Host-Range Plasmid pB10 in Sediment Microcosms by Quantitative PCR-Reference-Cited by-同舟云学术

Monitoring the Dissemination of the Broad-Host-Range Plasmid pB10 in Sediment Microcosms by Quantitative PCR

Published:2010-01 Issue:1 Volume:76 Page:378-382
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bonot Sébastien¹²,Merlin Christophe²

Affiliation:

1. Centre International de Recherche sur l'Eau et l'Environnement (CIRSEE), Suez Environnement, 38 Tue du Président Wilson, 78230 Le Pecq, France

2. LCPME, Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Nancy-Université, CNRS, 405 Rue de Vandoeuvre, 54600 Villers-lès-Nancy, France

Abstract

ABSTRACT Studying the transfer of specific mobile genetic elements in complex environmental matrices remains difficult because suitable molecular tools are not yet available to back up classical culture-dependent approaches. In this report, we show that quantitative PCR could be used to monitor the dissemination of the broad-host-range plasmid pB10 in sediment microcosms. This approach lies in the differential measurement of the host and plasmid DNAs used to inoculate the microcosms, using a particular design of quantitative PCR primers/probes where we took advantage of the mosaic aspect of the bacterial genomes to achieve a highly specific quantitative PCR detection system.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01125-09

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