Serologic and Molecular Characterization of Vibrio parahaemolyticus Strains Isolated from Seawater and Fish Products of the Gulf of Mexico-Reference-Cited by-同舟云学术

Serologic and Molecular Characterization of Vibrio parahaemolyticus Strains Isolated from Seawater and Fish Products of the Gulf of Mexico

Published:2004-11 Issue:11 Volume:70 Page:6401-6406
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Cabrera-García María Eugenia¹,Vázquez-Salinas Carlos²,Quiñones-Ramírez Elsa Irma¹

Affiliation:

1. Department of Microbiology of the National School of Biological Sciences, Instituto Politécnico Nacional

2. Department of Biotechnology, Division of Biological and Health Sciences, Universidad Autónoma Metropolitana, Mexico City, Mexico

Abstract

ABSTRACT The thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are the main virulence factors of Vibrio parahaemolyticus . We isolated V. parahaemolyticus from seawater, fish, and oysters obtained from the Pueblo Viejo Lagoon in Veracruz, determined the serogroups, phenotypically and genotypically characterized TDH and TRH, and investigated the presence of the toxR gene. A total of 46 V. parahaemolyticus strains were isolated, and all of them amplified the 368-bp toxR gene fragment. The trh gene was not identified in any of the strains; 4 of the 46 strains were Kanagawa phenomenon (KP) positive and amplified the 251-bp tdh gene fragment. The most frequent serogroup was serogroup O3. This is the first report of the presence of KP-positive tdh -positive environmental V. parahaemolyticus strains in Mexico.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.70.11.6401-6406.2004

Reference30 articles.

1. Emergence of a restricted bioserovar of Vibrio parahaemolyticus as the predominant cause of Vibrio-associated gastroenteritis on the West Coast of the United States and Mexico

2. Alam, M. J., K. Tomochika, S. Miyoshi, and S. Shinoda. 2002. Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan. FEMS Microbiol. Lett.208:83-87.

3. Castillo-Rivera, M., R. Zárate, and L. Sanvicente-Añorve. 2003. Patrones de la diversidad de peces en la laguna de Pueblo Viejo, Veracruz, México. Hidrobiologica13:289-298.

4. Vibrio parahaemolyticus Serovar O3:K6 as Cause of Unusually High Incidence of Food-Borne Disease Outbreaks in Taiwan from 1996 to 1999

5. Daniels, N. A., L. MacKinnon, R. Bishop, S. Altekruse, B. Ray, R. M. Hammond, S. Thompson, S. Wilson, N. H. Bean, P. M. Griffin, and L. Slutsker. 2000. Vibrio parahaemolyticus infection in the United States, 1973-1998. J. Infect. Dis.181:1661-1666.

Cited by 58 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Molecular characterization of Vibrio species isolated from dairy and water samples;Scientific Reports;2023-09-16

2. Molecular Characterization of <i>Vibrio parahaemolyticus</i> Isolates from Seawater, Fish Tanks, and Distributed Fishery Products in Jeju;Journal of Food Hygiene and Safety;2023-08-30

3. Development of a multiplex polymerase chain reaction (m-PCR) for the detection and identification of virulent and avirulent forms of Vibrio parahaemolyticus;Bionatura;2023-06-15

4. A lanthanide-based high-sensitivity fluorescence method for the on-site rapid detection of thermostable direct hemolysin of Vibrio parahaemolyticus;Journal of Food Protection;2023-01

5. Emergence and predominance of a new serotype of Vibrio parahaemolyticus in Huzhou, China;International Journal of Infectious Diseases;2022-09