Affiliation:
1. Département de Toxicologie, Centre de Recherches du Service de Santé des Armées, 38702 La Tronche Cedex, France
2. Département de Biologie des Agents Transmissibles
Abstract
ABSTRACT
Spores of
Bacillus anthracis
are known to be extremely resistant to heat treatment, irradiation, desiccation, and disinfectants. To determine inactivation kinetics of spores by high pressure,
B. anthracis
spores of a Sterne strain-derived mutant deficient in the production of the toxin components (strain RP42) were exposed to pressures ranging from 280 to 500 MPa for 10 min to 6 h, combined with temperatures ranging from 20 to 75°C. The combination of heat and pressure resulted in complete destruction of
B. anthracis
spores, with a
D
value (exposure time for 90% inactivation of the spore population) of approximately 4 min after pressurization at 500 MPa and 75°C, compared to 160 min at 500 MPa and 20°C and 348 min at atmospheric pressure (0.1 MPa) and 75°C. The use of high pressure for spore inactivation represents a considerable improvement over other available methods of spore inactivation and could be of interest for antigenic spore preparation.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference21 articles.
1. Bartlett, D. H. 2002. Pressure effects on in vivo microbial processes. Biochim. Biophys. Acta1595:367-381.
2. Anthrax Spores Make an Essential Contribution to Vaccine Efficacy
3. Initiation of Germination and Inactivation of
Bacillus pumilus
Spores by Hydrostatic Pressure
4. Delacour, H., C. Cléry, P. Masson, and D. R. Vidal. 2002. Inactivation des spores bactériennes par les hautes pressions hydrostatiques. Ann. Pharm. Fr.60:38-43.
5. Inglesby, T. V., T. O'Toole, D. A. Henderson, J. G. Bartlett, M. S. Ascher, E. Eitzen, A. M. Friedlander, J. Gerberding, J. Hauer, J. Hughes, J. McDade, M. T. Osterholm, G. Parker, T. M. Perl, P. K. Russell, and K. Tonat. 2002. Anthrax as a biological weapon, 2002. Updated recommendations for management. JAMA287:2236-2252.
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