Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification
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Published:2005-05
Issue:5
Volume:43
Page:2339-2344
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ISSN:0095-1137
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Container-title:Journal of Clinical Microbiology
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language:en
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Short-container-title:J Clin Microbiol
Author:
Wang Bin1, Potter Simon J.1, Lin Yiguang2, Cunningham Anthony L.1, Dwyer Dominic E.13, Su Yuelong4, Ma Xuejun4, Hou Yunde4, Saksena Nitin K.1
Affiliation:
1. Centre for Virus Research, Westmead Millennium Institute, The University of Sydney 2. Department of Health Sciences, University of Technology 3. CIDMLS, ICPMR, Westmead Hospital, Sydney, New South Wales, Australia 4. Beijing JDK Bio-technology Institute, National Institute of Virology, Chinese Academy of Preventive Medicine, Beijing, China
Abstract
ABSTRACT
The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the possibility of future epidemics. We describe here a rapid, sensitive, and highly efficient assay for the detection of SARS coronavirus (SARS-CoV) in cultured material and a small number (
n
= 7) of clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive detection levels of SARS-CoV RNA in both solid and liquid phases. The main advantage of RCA is that it can be performed under isothermal conditions with minimal reagents and avoids the generation of false-positive results, a problem that is frequently encountered in PCR-based assays. Furthermore, the RCA technology provides a faster, more sensitive, and economical option to currently available PCR-based methods.
Publisher
American Society for Microbiology
Subject
Microbiology (medical)
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