A Multi-Enzyme Cascade for Efficient Production of Pyrrolidone from <scp>l-</scp> Glutamate-Reference-Cited by-同舟云学术

A Multi-Enzyme Cascade for Efficient Production of Pyrrolidone from l- Glutamate

Published:2023-04-26 Issue:4 Volume:89 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Jiang Xuling¹²,Wei Wanqing²,Cui Yaozhong³,Song Wei¹,Li Yingying²,Chen Xiulai²^ORCID,Gao Cong²^ORCID,Liu Jia²,Guo Liang²,Liu Liming²,Wu Jing¹^ORCID

Affiliation:

1. School of Life Sciences and Health Engineering, Jiangnan University, Wuxi, China

2. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China

3. Jiangsu Xishan Senior High School, Wuxi, China

Abstract

This study developed a three-enzyme cascade pathway for the production of pyrrolidone from l- Glu. The catalytic efficiency of carnitine CoA ligase from Escherichia coli ( Ec CaiC) was improved by mechanism-based protein engineering, and the titer of pyrrolidone was further increased by ribosome-binding site (RBS), induction conditions, and conversion conditions optimization.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.00013-23

Reference42 articles.

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