Ecoepidemiology and Complete Genome Comparison of Different Strains of Severe Acute Respiratory Syndrome-Related
Rhinolophus
Bat Coronavirus in China Reveal Bats as a Reservoir for Acute, Self-Limiting Infection That Allows Recombination Events
-
Published:2010-03-15
Issue:6
Volume:84
Page:2808-2819
-
ISSN:0022-538X
-
Container-title:Journal of Virology
-
language:en
-
Short-container-title:J Virol
Author:
Lau Susanna K. P.1234, Li Kenneth S. M.4, Huang Yi4, Shek Chung-Tong5, Tse Herman1234, Wang Ming6, Choi Garnet K. Y.4, Xu Huifang6, Lam Carol S. F.4, Guo Rongtong6, Chan Kwok-Hung4, Zheng Bo-Jian4, Woo Patrick C. Y.1234, Yuen Kwok-Yung1234
Affiliation:
1. State Key Laboratory of Emerging Infectious Diseases 2. Research Centre of Infection and Immunology 3. Carol Yu Centre for Infection 4. Department of Microbiology, The University of Hong Kong, Hong Kong, Hong Kong 5. Department of Agriculture, Fisheries and Conservation, The Government of Hong Kong Special Administrative Region, Hong Kong, Hong Kong 6. Guangzhou Center for Disease Control and Prevention, Guangzhou, China
Abstract
ABSTRACT
Despite the identification of severe acute respiratory syndrome-related coronavirus (SARSr-CoV) in
Rhinolophus
Chinese horseshoe bats (SARSr-Rh-BatCoV) in China, the evolutionary and possible recombination origin of SARSr-CoV remains undetermined. We carried out the first study to investigate the migration pattern and SARSr-Rh-BatCoV genome epidemiology in Chinese horseshoe bats during a 4-year period. Of 1,401 Chinese horseshoe bats from Hong Kong and Guangdong, China, that were sampled, SARSr-Rh-BatCoV was detected in alimentary specimens from 130 (9.3%) bats, with peak activity during spring. A tagging exercise of 511 bats showed migration distances from 1.86 to 17 km. Bats carrying SARSr-Rh-BatCoV appeared healthy, with viral clearance occurring between 2 weeks and 4 months. However, lower body weights were observed in bats positive for SARSr-Rh-BatCoV, but not Rh-BatCoV HKU2. Complete genome sequencing of 10 SARSr-Rh-BatCoV strains showed frequent recombination between different strains. Moreover, recombination was detected between SARSr-Rh-BatCoV Rp3 from Guangxi, China, and Rf1 from Hubei, China, in the possible generation of civet SARSr-CoV SZ3, with a breakpoint at the nsp16/spike region. Molecular clock analysis showed that SARSr-CoVs were newly emerged viruses with the time of the most recent common ancestor (tMRCA) at 1972, which diverged between civet and bat strains in 1995. The present data suggest that SARSr-Rh-BatCoV causes acute, self-limiting infection in horseshoe bats, which serve as a reservoir for recombination between strains from different geographical locations within reachable foraging range. Civet SARSr-CoV is likely a recombinant virus arising from SARSr-CoV strains closely related to SARSr-Rh-BatCoV Rp3 and Rf1. Such frequent recombination, coupled with rapid evolution especially in ORF7b/ORF8 region, in these animals may have accounted for the cross-species transmission and emergence of SARS.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference54 articles.
1. Brandão, P. E., K. Scheffer, L. Y. Villarreal, S. Achkar, N. Oliveira Rde, O. Fahl Wde, J. G. Castilho, I. Kotait, and L. J. Richtzenhain. 2008. A coronavirus detected in the vampire bat Desmodus rotundus. Braz. J. Infect. Dis.12:466-468. 2. Brian, D. A., and R. S. Baric. 2005. Coronavirus genome structure and replication. Curr. Top. Microbiol. Immunol.287:1-30. 3. Carrington, C. V., J. E. Foster, H. C. Zhu, J. X. Zhang, G. J. Smith, N. Thompson, A. J. Auguste, V. Ramkissoon, A. A. Adesiyun, and Y. Guan. 2008. Detection and phylogenetic analysis of group 1 coronaviruses in South American bats. Emerg. Infect. Dis.14:1890-1893. 4. Chen, C. Y., Y. H. Ping, H. C. Lee, K. H. Chen, Y. M. Lee, Y. J. Chan, T. C. Lien, T. S. Jap, C. H. Lin, L. S. Kao, and Y. M. Chen. 2007. Open reading frame 8a of the human severe acute respiratory syndrome coronavirus not only promotes viral replication but also induces apoptosis. J. Infect. Dis.196:405-415. 5. Dominguez, S. R., T. J. O'Shea, L. M. Oko, and K. V. Holmes. 2007. Detection of group 1 coronaviruses in bats in North America. Emerg. Infect. Dis.13:1295-1300.
Cited by
229 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|