A Sampling of the Yeast Proteome-Reference-Cited by-同舟云学术

A Sampling of the Yeast Proteome

Published:1999-11 Issue:11 Volume:19 Page:7357-7368
ISSN:0270-7306
Container-title:Molecular and Cellular Biology
language:en
Short-container-title:Mol Cell Biol

Author:

Futcher B.¹,Latter G. I.¹,Monardo P.¹,McLaughlin C. S.²,Garrels J. I.³

Affiliation:

1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 1 ;

2. Department of Biological Chemistry, University of California, Irvine, California 92717 2 ; and

3. Proteome, Inc., Beverly, Massachusetts 019153

Abstract

ABSTRACT In this study, we examined yeast proteins by two-dimensional (2D) gel electrophoresis and gathered quantitative information from about 1,400 spots. We found that there is an enormous range of protein abundance and, for identified spots, a good correlation between protein abundance, mRNA abundance, and codon bias. For each molecule of well-translated mRNA, there were about 4,000 molecules of protein. The relative abundance of proteins was measured in glucose and ethanol media. Protein turnover was examined and found to be insignificant for abundant proteins. Some phosphoproteins were identified. The behavior of proteins in differential centrifugation experiments was examined. Such experiments with 2D gels can give a global view of the yeast proteome.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MCB.19.11.7357

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