Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-Density Microarray-Reference-Cited by-同舟云学术

Generic Detection of Coronaviruses and Differentiation at the Prototype Strain Level by Reverse Transcription-PCR and Nonfluorescent Low-Density Microarray

Published:2007-03 Issue:3 Volume:45 Page:1049-1052
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

de Souza Luna Luciano Kleber¹,Heiser Volker²,Regamey Nicolas³,Panning Marcus¹,Drexler Jan Felix¹,Mulangu Sabue⁴,Poon Leo⁵,Baumgarte Sigrid⁶,Haijema Bert Jan⁷,Kaiser Laurent⁸,Drosten Christian¹

Affiliation:

1. Clinical Virology Section, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany

2. Chipron GmbH, Berlin, Germany

3. National Heart and Lung Institute, Imperial College London, United Kingdom

4. Institut National de Recherche Biomédicale, Kinshasa, Democratic Republic of Congo

5. Department of Microbiology, University of Hong Kong, China

6. Institute of Hygiene and Environmental Health, Hamburg, Germany

7. Institute of Virology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands

8. Central Laboratory of Virology, University Hospitals of Geneva, Switzerland

Abstract

ABSTRACT A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02426-06

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