Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease-Reference-Cited by-同舟云学术

Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease

Published:2022-08-17 Issue:8 Volume:60 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

de Rezende Izabela Maurício¹²^ORCID,Oliveira Gabriela Fernanda Garcia¹,Costa Thaís Alkifeles¹,Khan Aslam²,Pereira Leonardo Soares¹³,Santos Tayrine Araújo³,Alves Pedro Augusto⁴,Calzavara-Silva Carlos Eduardo⁵,Martins-Filho Olindo Assis⁶,Teixeira-Carvalho Andréa⁶,LaBeaud Angelle Desiree²,Drumond Betânia Paiva¹^ORCID

Affiliation:

1. Laboratory of Viruses, Microbiology Department, Biological Sciences Institute, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

2. Department of Pediatrics, Division of Infectious Disease, Stanford University School of Medicine, Stanford, California, USA

3. Eduardo de Menezes Hospital, Belo Horizonte, Minas Gerais, Brazil

4. Immunology of Viruses Diseases, René Rachou Institute, Oswaldo Cruz Foundation/FIOCRUZ MG, Belo Horizonte, Minas Gerais, Brazil

5. Cellular and Molecular Immunology, René Rachou Institute, Oswaldo Cruz Foundation/FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil

6. Integrated Group of Biomarkers Research, René Rachou Institute, Oswaldo Cruz Foundation/FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil

Abstract

Prior studies have demonstrated prolonged presence of yellow fever virus (YFV) RNA in saliva and urine as an alternative to serum. To investigate the presence of YFV RNA in urine, we used RT-PCR for YFV screening in 60 urine samples collected from a large cohort of naturally infected yellow fever (YF) patients during acute and convalescent phases of YF infection from recent YF outbreaks in Brazil (2017 to 2018).

Funder

Fundação de Amparo à Pesquisa do Estado de Minas Gerais

HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases

Fundação Oswaldo Cruz

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

MCTI | Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.00254-22