Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2

Author:

Liu Wanbing1,Liu Lei1,Kou Guomei1,Zheng Yaqiong1,Ding Yinjuan1,Ni Wenxu1,Wang Qiongshu2,Tan Li2,Wu Wanlei1,Tang Shi1,Xiong Zhou1,Zheng Shangen1

Affiliation:

1. Department of Transfusion, General Hospital of Central Theater Command of the People’s Liberation Army, Wuhan, Hubei, China

2. Department of Disease Control and Prevention, General Hospital of Central Theater Command of the People’s Liberation Army, Wuhan, Hubei, China

Abstract

At present, PCR-based nucleic acid detection cannot meet the demands for coronavirus infectious disease (COVID-19) diagnosis. Two hundred fourteen confirmed COVID-19 patients who were hospitalized in the General Hospital of Central Theater Command of the People’s Liberation Army between 18 January and 26 February 2020 were recruited. Two enzyme-linked immunosorbent assay (ELISA) kits based on recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (rN) and spike protein (rS) were used for detecting IgM and IgG antibodies, and their diagnostic feasibility was evaluated.

Funder

China Postdoctoral Science Foundation

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference20 articles.

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