Detection of Ehrlichia spp. in the Blood of Wild White-Tailed Deer in Missouri by PCR Assay and Serologic Analysis

Author:

Arens Max Q.1,Liddell Allison M.2,Buening Gerald3,Gaudreault-Keener Monique4,Sumner John W.5,Comer James A.5,Buller Richard S.1,Storch Gregory A.126

Affiliation:

1. Department of Pediatrics, Washington University School of Medicine and St. Louis Children's Hospital

2. Departments of Medicine

3. Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri

4. Clinical Virology Laboratory, St. Louis Children's Hospital

5. Centers for Disease Control and Prevention, Atlanta, Georgia

6. Molecular Microbiology, Washington University School of Medicine, St. Louis

Abstract

ABSTRACT Blood samples collected from wild deer in Missouri in November of 2000 and 2001 were positive by PCR assays for Ehrlichia chaffeensis (50 of 217; 23%), Ehrlichia ewingii (44 of 217; 20%), and Anaplasma species (214 of 217; 99%). Nucleotide sequences of selected amplicons from the assay for anaplasma matched sequences of the white-tailed deer agent. Serologic analysis of 112 deer sampled in 2000 showed a very high prevalence of antibodies to E . chaffeensis (97 of 112; 87%) and a low prevalence of antibodies reactive with Anaplasma phagocytophila (2 of 112; 2%).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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