Use of Pulsed-Field Gel Electrophoresis of Conserved XbaI Fragments for Identification of Swine Salmonella Serotypes-Reference-Cited by-同舟云学术

Use of Pulsed-Field Gel Electrophoresis of Conserved XbaI Fragments for Identification of Swine Salmonella Serotypes

Published:2007-02 Issue:2 Volume:45 Page:472-476
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Gaul Stephen B.¹,Wedel Stephanie¹,Erdman Matthew M.¹,Harris D. L.¹²,Harris Isabel Turney¹,Ferris Kathleen E.³,Hoffman Lorraine²

Affiliation:

1. Department of Animal Science

2. Veterinary Diagnostic and Production Animal Medicine, Iowa State University

3. National Veterinary Services Laboratory, Ames, Iowa

Abstract

ABSTRACT Swine Salmonella isolates ( n = 674) from various locations throughout the United States and Canada were analyzed via pulsed-field gel electrophoresis (PFGE) with XbaI. PFGE subtypes were analyzed by cluster analysis and compared to conventional serotyping results. The analysis showed a correlation of serotype to PFGE subtype. In addition, conserved fragments were identified within the restriction patterns that were unique to each serotype. PFGE using XbaI restriction provided a possible alternative method for screening and identifying swine Salmonella serotypes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00962-06

Reference17 articles.

1. Profile of Salmonella enterica subsp. enterica (Subspecies I) Serotype 4,5,12:i:− Strains Causing Food-Borne Infections in New York City

2. Combined Use of Two Genetic Fingerprinting Methods, Pulsed-Field Gel Electrophoresis and Ribotyping, for Characterization of Escherichia coli O157 Isolates from Food Animals, Retail Meats, and Cases of Human Disease

3. Bender, J. B., C. W. Hedberg, J. M. Besser, J. H. Boxrud, J. H. Wicklund, and M. T. Osterholm. 1998. Surveillance for Salmonella typhimurium infections in Minnesota by molecular subtype, Abstr. Int. Conf. Emerg. Infect. Dis., p. 116.

4. Bender, J. B., C. W. Hedberg, D. J. Boxrud, J. M. Besser, J. H. Wicklund, K. E. Smith, and M. T. Osterholm. 2001. Use of molecular subtyping in surveillance for Salmonella enterica serotype typhimurium. N. Engl. J. Med.344:189-195.

5. Cloeckaert, A., and S. Schwarz. 2001. Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104. Vet. Res.32:301-310.

Cited by 35 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A large-scale surveillance revealed that KPC variants mediated ceftazidime-avibactam resistance in clinically isolated Klebsiella pneumoniae;Microbiology Spectrum;2024-08-06

2. Assessing the mechanisms of multi-drug resistant non-typhoidal Salmonella (NTS) serovars isolated from layer chicken farms in Nigeria;PLOS ONE;2023-09-07

3. Phenotypic and Genetic Comparison of a Plant-Internalized and an Animal-Isolated Salmonella Choleraesuis Strain;Microorganisms;2021-07-21

4. Assessment and Comparison of Molecular Subtyping and Characterization Methods for Salmonella;Frontiers in Microbiology;2019-07-12

5. Pulsed-field gel electrophoresis fingerprinting of Campylobacter jejuni and Campylobacter coli strains isolated from clinical specimens, Iran;International Microbiology;2019-03-14