Coexistence of Two bla NDM-5 Genes on an IncF Plasmid as Revealed by Nanopore Sequencing-Reference-Cited by-全球学者库

Coexistence of Two bla NDM-5 Genes on an IncF Plasmid as Revealed by Nanopore Sequencing

Published:2018-05 Issue:5 Volume:62 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Feng Yu¹²,Liu Lu¹²,McNally Alan³,Zong Zhiyong¹²⁴⁵

Affiliation:

1. Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China

2. Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China

3. Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom

4. Department of Infection Control, West China Hospital, Sichuan University, Chengdu, China

5. Center for Pathogen Research, West China Hospital, Sichuan University, Chengdu, China

Abstract

ABSTRACT In a carbapenem-resistant Escherichia coli clinical isolate of sequence type 167, two copies of bla NDM-5 were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B − type. Both bla NDM-5 genes were located in 11,065-bp regions flanked by two copies of IS 26 . The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts of bla NDM-5 .

Funder

National Natural Science Foundation of China

Royal Society

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00110-18

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