Rapid biochemical tests for characterization of the Mycoplasmatales-Reference-Cited by-同舟云学术

Rapid biochemical tests for characterization of the Mycoplasmatales

Published:1977-05 Issue:5 Volume:5 Page:531-534
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bradbury J M

Abstract

Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.5.5.531-534.1977

Reference9 articles.

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2. Simple method for immunofluorescent identification of mycoplasma colonies;Bradbury J. M.;J. Clin. Microbiol.,1976

3. Bovine mycoplasmas: cultural and biochemical studies II;Erno H.;Acta Vet. Scand.,1973

4. Importance of extension and standardization of laboratory tests for the identification and classification of mycoplasma;Fabricant J.;Am. N. Y. Acad. Sci.,1967

5. Fluorometric assay for the hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone;Jacks T. J.;Anal. Biochem.,1967

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