Affiliation:
1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
2. Department of Medical Microbiology and Infectious Disease, Cardiff Institute of Infection & Immunity, Heath Park Hospital, Cardiff, United Kingdom
Abstract
ABSTRACT
Carbapenem-resistant
Enterobacteriaceae
(CRE) have spread worldwide, leaving very few treatment options available. New Delhi metallo-beta-lactamase (NDM) is the main carbapenemase mediating CRE resistance and is of increasing concern. NDM-positive
Enterobacteriaceae
of human origin are frequently identified; however, the emergence of NDM, and particularly novel variants, in bacteria of food animal origin has never been reported. Here, we characterize a novel NDM variant (assigned NDM-17) identified in a β-lactam-resistant sequence type 48 (ST48)
Escherichia coli
strain that was isolated from a chicken in China. Compared to NDM-1, NDM-17 had three amino acid substitutions (V88L, M154L, and E170K) that confer significantly enhanced carbapenemase activity. Compared to NDM-5, NDM-17 had only one amino acid substitution (E170K) and slightly increased isolate resistance to carbapenem, as indicated by increased MIC values. The gene encoding NDM-17 (
bla
NDM-17
) was located on an IncX3 plasmid, which was readily transferrable to recipient
E. coli
strain J53 by conjugation, suggesting the possibility of the rapid dissemination of
bla
NDM-17
. Enzyme kinetics showed that NDM-17 could hydrolyze all β-lactams tested, except for aztreonam, and had a significantly higher affinity for all β-lactams tested than did NDM-5. The emergence of this novel NDM variant could pose a threat to public health because of its transferability and enhanced carbapenemase activity.
Funder
National Basic Research Program of China
National Natural Science Foundation of China
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
60 articles.
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