A Whole-Genome-Based Gene-by-Gene Typing System for Standardized High-Resolution Strain Typing of Bacillus anthracis

Author:

Abdel-Glil Mostafa Y.12ORCID,Chiaverini Alexandra3,Garofolo Giuliano3ORCID,Fasanella Antonio4,Parisi Antonio4,Harmsen Dag5,Jolley Keith A.6,Elschner Mandy C.1,Tomaso Herbert1,Linde Jörg1,Galante Domenico4

Affiliation:

1. Institute for Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, Jena, Germany

2. Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkia Province, Egypt

3. Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy

4. Anthrax Reference Institute of Italy, Istituto Zooprofilattico Sperimentale della Puglia e Basilicata, Foggia, Italy

5. Department of Periodontology and Operative Dentistry, University Hospital Muenster, Muenster, Germany

6. Department of Zoology, University of Oxford, Oxford, United Kingdom

Abstract

Whole-genome sequencing (WGS) has been established for bacterial subtyping and is regularly used to study pathogen transmission, to investigate outbreaks, and to perform routine surveillance. Core-genome multilocus sequence typing (cgMLST) is a bacterial subtyping method that uses WGS data to provide a high-resolution strain characterization. This study aimed at developing a novel cgMLST scheme for Bacillus anthracis , a notorious pathogen that causes anthrax in livestock and humans worldwide.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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