Temperature Compensation in Methanosarcina barkeri by Modulation of Hydrogen and Acetate Affinity

Author:

Westermann Peter1,Ahring Birgitte K.1,Mah Robert A.1

Affiliation:

1. Environmental and Occupational Health Sciences, School of Public Health, University of California, Los Angeles, California 90024

Abstract

The affinity of Methanosarcina barkeri 227 for acetate and hydrogen at different incubation temperatures was investigated. Increasing the temperature from 20 to 37�C resulted in a 4.5-fold increase in K m for acetate and a 4.8-fold increase for hydrogen. The corresponding increase in V max for acetate was 8.3-fold (5.4-fold for hydrogen). This response implied a decrease in the temperature coefficient (Q 10 ) and hence a decrease in the temperature dependency as a function of decreasing substrate concentration.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference29 articles.

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