Two New Complete Genome Sequences Offer Insight into Host and Tissue Specificity of Plant Pathogenic Xanthomonas spp

Author:

Bogdanove Adam J.1,Koebnik Ralf2,Lu Hong3,Furutani Ayako45,Angiuoli Samuel V.6,Patil Prabhu B.7,Van Sluys Marie-Anne89,Ryan Robert P.10,Meyer Damien F.111,Han Sang-Wook12,Aparna Gudlur1314,Rajaram Misha1516,Delcher Arthur L.617,Phillippy Adam M.618,Puiu Daniela6,Schatz Michael C.619,Shumway Martin20,Sommer Daniel D.6,Trapnell Cole121,Benahmed Faiza2223,Dimitrov George22,Madupu Ramana22,Radune Diana22,Sullivan Steven2224,Jha Gopaljee1325,Ishihara Hiromichi826,Lee Sang-Won1227,Pandey Alok13,Sharma Vikas7,Sriariyanun Malinee12,Szurek Boris2,Vera-Cruz Casiana M.28,Dorman Karin S.15,Ronald Pamela C.12,Verdier Valérie2,Dow J. Maxwell10,Sonti Ramesh V.13,Tsuge Seiji29,Brendel Volker P.315,Rabinowicz Pablo D.221730,Leach Jan E.8,White Frank F.31,Salzberg Steven L.632

Affiliation:

1. Department of Plant Pathology, Iowa State University, Ames, Iowa 50011

2. Institut de Recherche pour le Développement, UMR RPB IRD-CIRAD-UM2, 911 Avenue Agropolis BP 64501, 34394 Montpellier Cedex 5, France

3. Department of Genetics Cell and Developmental Biology, Iowa State University, Ames, Iowa 50011

4. Department of Genetic Resources, National Institute of Agrobiological Sciences, Kannondai, Tsukuba 305-8602, Japan

5. Gene Research Center, Ibaraki University, Ami, Ibaraki 300-0393, Japan

6. Center for Bioinformatics and Computational Biology, University of Maryland, College Park, Maryland 20742

7. Institute of Microbial Technology, Council of Scientific and Industrial Research, Sector 39A, Chandigarh 160036, India

8. Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, Colorado 80523

9. Departamento de Botânica, IB-USP, Sao Paulo, SP, Brazil

10. BIOMERIT Research Centre, BioSciences Institute, University College Cork, Cork, Ireland

11. CIRAD/INRA, Control of Emerging and Exotic Animal Diseases, Domaine de Duclos, Prise d'Eau 97170 Petit-Bourg, Guadeloupe, France

12. Department of Plant Pathology, University of California, Davis, California 95616

13. Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad, India

14. La Jolla Institute for Allergy and Immunology, La Jolla, California 92037

15. Department of Statistics, Iowa State University, Ames, Iowa 50011

16. Cardiovascular Research Institute, University of California, San Francisco, San Francisco, California 94143

17. Institute for Genome Sciences (IGS), University of Maryland School of Medicine, Baltimore, Maryland 21201

18. National Biodefense Analysis and Countermeasures Center, Frederick, Maryland 21702

19. Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

20. National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland 20892

21. Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts 02138

22. The Institute for Genomic Research, Rockville, Maryland 20850

23. Division of Food and Animal Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708

24. Center for Genomics and Systems Biology, New York University, 1009 Silver Building, 100 Washington Square East, New York, New York 10003

25. The Institute of Himalayan Bioresouce Technology, Council of Scientific and Industrial Research, Palampur, India

26. Department of Material Chemistry, Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 3-1-1, Okayama 700-8530, Japan

27. Department of Plant Molecular Systems Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 446-701, South Korea

28. Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

29. Laboratory of Plant Pathology, Kyoto Prefectural University, Sakyo, Kyoto 606-8522 Japan

30. Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201

31. Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506

32. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Abstract

ABSTRACT Xanthomonas is a large genus of bacteria that collectively cause disease on more than 300 plant species. The broad host range of the genus contrasts with stringent host and tissue specificity for individual species and pathovars. Whole-genome sequences of Xanthomonas campestris pv. raphani strain 756C and X. oryzae pv. oryzicola strain BLS256, pathogens that infect the mesophyll tissue of the leading models for plant biology, Arabidopsis thaliana and rice, respectively, were determined and provided insight into the genetic determinants of host and tissue specificity. Comparisons were made with genomes of closely related strains that infect the vascular tissue of the same hosts and across a larger collection of complete Xanthomonas genomes. The results suggest a model in which complex sets of adaptations at the level of gene content account for host specificity and subtler adaptations at the level of amino acid or noncoding regulatory nucleotide sequence determine tissue specificity.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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