Simultaneous Detection and Discrimination of Virulent and Benign Dichelobacter nodosus in Sheep of Flocks Affected by Foot Rot and in Clinically Healthy Flocks by Competitive Real-Time PCR-Reference-Cited by-同舟云学术

Simultaneous Detection and Discrimination of Virulent and Benign Dichelobacter nodosus in Sheep of Flocks Affected by Foot Rot and in Clinically Healthy Flocks by Competitive Real-Time PCR

Published:2014-04 Issue:4 Volume:52 Page:1228-1231
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Stäuble Anna¹²,Steiner Adrian²,Frey Joachim¹,Kuhnert Peter¹

Affiliation:

1. Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland

2. Clinic for Ruminants, Vetsuisse Faculty, University of Bern, Bern, Switzerland

Abstract

ABSTRACT Ovine foot rot caused by Dichelobacter nodosus is affecting sheep worldwide. The current diagnostic methods are difficult and cumbersome. Here, we present a competitive real-time PCR based on allelic discrimination of the protease genes aprV2 and aprB2 . This method allows direct detection and differentiation of virulent and benign D. nodosus from interdigital skin swabs in a single test. Clinically affected sheep harbored high loads of only virulent strains, whereas healthy sheep had lower loads of predominantly benign strains.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.03485-13

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5. DETECTION OF DICHELOBACTER NODOSUS IN WILD UNGULATES (CAPRA IBEX IBEX AND OVIS ARIES MUSIMON) AND DOMESTIC SHEEP SUFFERING FROM FOOT ROT USING A TWO-STEP POLYMERASE CHAIN REACTION

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