Application of tRNA Intergenic Spacer PCR for Identification of Enterococcus Species-Reference-Cited by-同舟云学术

Application of tRNA Intergenic Spacer PCR for Identification of Enterococcus Species

Published:2000-11 Issue:11 Volume:38 Page:4201-4207
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Baele Margo¹,Baele Paul¹,Vaneechoutte Mario²,Storms Virginie³,Butaye Patrick¹,Devriese Luc A.¹,Verschraegen Gerda²,Gillis Monique³,Haesebrouck Freddy¹

Affiliation:

1. Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, University of Ghent, B-9820 Merelbeke,1

2. Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine, University of Ghent,2 and

3. Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent,3 B-9000 Ghent, Belgium

Abstract

ABSTRACT tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faecium showed minor but reproducible differences with Enterococcus durans , while Enterococcus hirae was easily distinguishable. Enterococcus avium , Enterococcus malodoratus , and Enterococcus raffinosus generated highly similar though distinctive patterns.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.11.4201-4207.2000

Reference29 articles.

1. Proficiency of Clinical Laboratories in Spain in Detecting Vancomycin-Resistant Enterococcus spp

2. Identification of clinically relevant viridans streptococci by analysis of transfer DNA intergenic spacer length polymorphism;Degheldre Y.;Int. J. Syst. Bacteriol.,1999

3. Evaluation of arbitrarily primed PCR analysis and pulsed-field gel electrophoresis of large genomic DNA fragments for identification of enterococci important in human medicine;Descheemaeker P.;Int. J. Syst. Bacteriol.,1997

4. Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups;Devriese L. A.;J. Appl. Bacteriol.,1993

5. Acidification of methyl-alpha-D-glucopyranoside: a useful test to differentiate Enterococcus casseliflavus and Enterococcus gallinarum from Enterococcus faecium species group and from Enterococcus faecalis

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