Application of tRNA Intergenic Spacer PCR for Identification of Enterococcus Species

Author:

Baele Margo1,Baele Paul1,Vaneechoutte Mario2,Storms Virginie3,Butaye Patrick1,Devriese Luc A.1,Verschraegen Gerda2,Gillis Monique3,Haesebrouck Freddy1

Affiliation:

1. Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, University of Ghent, B-9820 Merelbeke,1

2. Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine, University of Ghent,2 and

3. Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent,3 B-9000 Ghent, Belgium

Abstract

ABSTRACT tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faecium showed minor but reproducible differences with Enterococcus durans , while Enterococcus hirae was easily distinguishable. Enterococcus avium , Enterococcus malodoratus , and Enterococcus raffinosus generated highly similar though distinctive patterns.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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