Viral Replicase Gene Products Suffice for Coronavirus Discontinuous Transcription-Reference-Cited by-同舟云学术

Viral Replicase Gene Products Suffice for Coronavirus Discontinuous Transcription

Published:2001-07-15 Issue:14 Volume:75 Page:6676-6681
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Thiel Volker¹,Herold Jens¹,Schelle Barbara¹,Siddell Stuart G.¹

Affiliation:

1. Institute of Virology and Immunology, University of Würzburg, 97078 Würzburg, Germany

Abstract

ABSTRACT We have used vaccinia virus as a vector to clone a 22.5-kbp cDNA that represents the 5′ and 3′ ends of the human coronavirus 229E (HCoV 229E) genome, the HCoV 229E replicase gene, and a single reporter gene (coding for green fluorescent protein [GFP]) located downstream of a regulatory element for coronavirus mRNA transcription. When RNA transcribed from this cDNA was transfected into BHK-21 cells, a small percentage of cells displayed strong fluorescence. A region of the mRNA encoding GFP was amplified by PCR and shown to have the unique mRNA leader-body junction indicative of coronavirus-mediated transcription. These data show that the coronavirus replicase gene products suffice for discontinuous subgenomic mRNA transcription.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.75.14.6676-6681.2001

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