Affiliation:
1. Department
of Biological Sciences, University of Essex, Colchester CO4 3SQ, United
Kingdom
2. California
Department of Health Services, Richmond,
California
Abstract
ABSTRACT
Human
parechoviruses (HPeV), members of the
Parechovirus
genus of
Picornaviridae
, are frequent pathogens but have been
comparatively poorly studied, and little is known of their diversity,
evolution, and molecular biology. To increase the amount of information
available, we have analyzed 7 HPeV strains isolated in California
between 1973 and 1992. We found that, on the basis of VP1 sequences,
these fall into two genetic groups, one of which has not been
previously observed, bringing the number of known groups to five. While
these correlate partly with the three known serotypes, two members of
the HPeV2 serotype belong to different genetic groups. In view of the
growing importance of molecular techniques in diagnosis, we suggest
that genotype is an important criterion for identifying viruses, and we
propose that the genetic groups we have defined should be termed human
parechovirus types 1 to 5. Complete nucleotide sequence analysis of two
of the Californian isolates, representing two types, confirmed the
identification of a new genetic group and suggested a role for
recombination in parechovirus evolution. It also allowed the
identification of a putative HPeV1
cis
-acting replication
element, which is located in the VP0 coding region, as well as the
refinement of previously predicted 5′ and 3′
untranslated region structures. Thus, the results have significantly
improved our understanding of these common
pathogens.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
123 articles.
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