Real-Time Nanopore Q20+ Sequencing Enables Extremely Fast and Accurate Core Genome MLST Typing and Democratizes Access to High-Resolution Bacterial Pathogen Surveillance-Reference-Cited by-同舟云学术

Real-Time Nanopore Q20+ Sequencing Enables Extremely Fast and Accurate Core Genome MLST Typing and Democratizes Access to High-Resolution Bacterial Pathogen Surveillance

Published:2023-04-20 Issue:4 Volume:61 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Wagner Gabriel E.¹^ORCID,Dabernig-Heinz Johanna¹,Lipp Michaela¹,Cabal Adriana²^ORCID,Simantzik Jonathan³,Kohl Matthias³,Scheiber Martina¹,Lichtenegger Sabine¹,Ehricht Ralf⁴⁵⁶,Leitner Eva¹,Ruppitsch Werner²^ORCID,Steinmetz Ivo¹^ORCID

Affiliation:

1. Diagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria

2. Austrian Agency for Health and Food Safety, Vienna, Austria

3. Medical and Life Sciences Faculty, Furtwangen University, Villingen-Schwenningen, Germany

4. InfectoGnostics Research Campus, Centre for Applied Research, Jena, Germany

5. Leibniz-Institute of Photonic Technology (Leibniz-IPHT), Jena, Germany

6. Friedrich Schiller University Jena, Institute of Physical Chemistry, Jena, Germany

Abstract

Next-generation whole-genome sequencing is essential for high-resolution surveillance of bacterial pathogens, for example, during outbreak investigations or for source tracking and escape variant analysis. However, current global sequencing and bioinformatic bottlenecks and a long time to result with standard technologies demand new approaches.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.01631-22

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