Affiliation:
1. Walter Reed Army Institute of Research, Washington, D.C. 20012
Abstract
The genes controlling synthesis of
Shigella flexneri
group- and type-specific antigens were transferred to
Escherichia coli
K-12 recipients by conjugation with an
S. flexneri
Hfr. After mating
E. coli
with an Hfr strain of
S. flexneri
2a and selecting for
his
+
recombinants, a high proportion of the
E. coli
hybrids agglutinated in
S. flexneri
grouping serum. None of these hybrids expressed
S. flexneri
type-specific antigen II. When an
E. coli his
+
hybrid possessing the
S. flexneri
group antigen was remated with the same Hfr with selection for
pro
+
hybrids, a high proportion now expressed the type-specific antigen as well as the previously inherited group antigen. If such crosses were performed in reverse order (i.e.,
pro
+
followed by
his
+
selection), a different pattern of serological behavior was observed. None of the
pro
+
hybrids showed the type-specific antigen. Subsequent mating for
his
+
resulted in hybrids with both the group- and type-specific antigens. These results show that genes controlling the synthesis of
S. flexneri
group antigen (linked to the
his
locus) and type-specific antigen (linked to the
pro
locus) are widely separated on the chromosome. Expression of the type-specific antigen II depends on the presence of the group antigen.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
54 articles.
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