Affiliation:
1. Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Abstract
We used cells carrying plasmids causing the overproduction of Gln3p, Ure2p, or both of these proteins to elucidate the ability of Ure2p to prevent the activation of gene expression by Gln3p in cells growing in a glutamine-containing medium. Our results indicate that Ure2p probably does not interfere with the binding of the GATA factor Gln3p to GATAAG sites but acts directly on Gln3p to block its ability to activate transcription.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference8 articles.
1. Recognition of nitrogen-responsive upstream activation sequences of Saccharomyces cerevisiae by the product of the GLN3 gene;Blinder D.;J. Bacteriol.,1995
2. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene;Broach J. R.;Gene,1979
3. Coschigano P. W. 1991. Ph.D. thesis. Massachusetts Institute of Technology Cambridge.
4. The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione S-transferases;Coschigano P. W.;Mol. Cell. Biol.,1991
5. Yeast mutants pleiotropically impaired in the regulation of two glutamate dehydrogenases;Drillien R.;Biochem. Biophys. Res. Commun.,1973
Cited by
104 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献