Changes in the Length of the Neuraminidase Stalk Region Impact H7N9 Virulence in Mice-Reference-Cited by-全球学者库

Changes in the Length of the Neuraminidase Stalk Region Impact H7N9 Virulence in Mice

Published:2016-02-15 Issue:4 Volume:90 Page:2142-2149
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Bi Yuhai¹²³,Xiao Haixia⁴³,Chen Quanjiao⁵³,Wu Yan¹,Fu Lifeng¹,Quan Chuansong⁶,Wong Gary¹,Liu Jun³⁶,Haywood Joel¹,Liu Yingxia²,Zhou Boping²,Yan Jinghua¹,Liu Wenjun¹³,Gao George F.¹²⁴³⁶

Affiliation:

1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

2. Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen, China

3. Center for Influenza Research and Early Warning (CASCIRE), Chinese Academy of Sciences, Beijing, China

4. Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China

5. CAS Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Hubei, China

6. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, China

Abstract

ABSTRACT The neuraminidase stalk of the newly emerged H7N9 influenza virus possesses a 5-amino-acid deletion. This study focuses on characterizing the biological functions of H7N9 with varied neuraminidase stalk lengths. Results indicate that the 5-amino-acid deletion had no impact on virus infectivity or replication in vitro or in vivo compared to that of a virus with a full-length stalk, but enhanced virulence in mice was observed for H7N9 encoding a 19- to 20-amino-acid deletion, suggesting that N9 stalk length impacts virulence in mammals, as N1 stalk length does.

Funder

China Ministry of Science and Technology Project 973

Tianjin Research Program of the Application Foundation and Advanced Technology

Influenza Virus Research of the Chinese Academy of Sciences

Open Research Fund Program of the CAS Key Laboratory of Special Pathogens and Biosafety

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.02553-15